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JB LAB

Dr. Johannes Broichhagen
Junior Group Leader
Leibniz-FMP Berlin

 

RESEARCH

 
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DEUTERATED RHODAMINES

We develop and use "d12" deuterium congeners of rhodamines that exhibit higher brightness and longer lifetimes with reduced bleaching versus their parent fluorophores TMR and SiR. Have a look at improved Förster resonance energy transfer (FRET), fluorescence activated cell sorting (FACS), fluorescence lifetime microscopy (FLIM) and stimulated emission depletion (STED) nanoscopy in our latest preprint.

[Roßmann et al., bioRxiv 2020]

MEMBERS

JOHANNES BROICHHAGEN

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Group Leader

JB studied chemistry at the University of Erlangen-Nuremberg including a one-year stint with Marcus Weck at NYU and graduated in 2010 with honors. He then joined the group of Dirk Trauner at LMU Munich to dive into Chemical Biology and obtained his PhD in 2014. Next, he was working with Kai Johnsson at EPF Lausanne and at the MPI for Medical Research in Heidelberg. Since March 2020, he joined the FMP in Berlin as a Junior Group Leader.

RAMONA BIRKE

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Technical Assistant

Ramona studied Biology with a focus on genetics and molecular biology at the Humboldt University Berlin, graduating 2010 in Molecular Ecology. After a stint at WITA GmbH, she joined the FMP as technical assistant in 2011, working in the field Molecular Cellular Physiology and Mass Spectrometry. Enjoying nature and being a passionate hiker in her free time, she teamed up with JB in 2020 to support and pursue our quests in Chemical Biology.

KILIAN ROSSMANN

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PhD Student

Kilian studied chemistry at the Julius-Maximilian-University Würzburg (JMU) with focus on Organic, Physical and Medicinal Chemistry. For his Master Thesis he joined the group of Dirk Trauner at New York University (NYU), where he worked on photoswitchable PROTACs. After graduating in 2019, Kilian decided to join JB’s lab in 2020 to work on novel imaging techniques.

JAHAZIEL JAHZERAH

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PhD Student

Jahzy obtained a BSc in chemistry and chemical engineering from the University of Wisconsin ‑ Madison. After moving to Berlin he shifted his focus to synthetic chemical biology and completed his master’s degree under the supervision of Christian Hackenberger. Since October 2020 he is working on a collaboration between JB’s lab and the the Liu lab.

CHRISTIANE HUHN

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Master's Thesis

Christiane obtained a BSc in chemistry from the Humboldt-University of Berlin in 2019. She joined the JB lab in October 2021 for her Master's Thesis.

ELDA SANCHEZ

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Erasmus Student

Elda obtained a BSc in biotechnology with a focus on biomedicine and pharmacology from the University of Cádiz (Spain) in 2021. She joined the JB lab in October 2021 for an Erasmus internship until March 2022.

Photos by Silke Oßwald.

 

OPEN POSITIONS

  • Internships (>12 weeks) for B.Sc./M.Sc. students available

We are always looking for motivated and enthusiastic people!

Interested? Send an email to JB with a letter of motivation and your CV.

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PREPRINTS

5.     Mugabo, Y.; Zhao, C.; Tan, J. J.; Ghosh, A.; Campbell, S. A.; Fadzeyeva, E.; Paré, F.; Pan, S. S.; Galipeau, M.; Ast, J.; Broichhagen, J.; Hodson, D. J.; Mulvihill, E. E.; Petropoulos, S.; Lim, G. E., 14-3-3ζ constrains insulin secretion by regulating mitochondrial function in pancreatic β-cells, bioRxiv 2021, https://doi.org/10.1101/2021.10.17.464702.


4.     An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo, Ast, J.; Novak, A. N.; Podewin, P.; Fine, N. H. F.; Jones, B.; Tomas, A.; Birke, R.; Roßmann, K.; Mathes, B.; Eichhorst, J.; Lehmann, M.; Linnemann, A. K.; Hodson, D. J.; Broichhagen, J.*, ChemRxiv 2021, https://doi.org/10.33774/chemrxiv-2021-7rngq.

3. Sulfonated rhodamines as impermeable labelling substrates for cell surface protein visualization, Birke, R.; Ast, J.; Roosen, D. A.; Mathes, B.; Roßmann, K.; Huhn, C.; Jones, B.; Lehmann, M.; Haucke, V.; Hodson, D. J.; Broichhagen, J.bioRxiv 2021

https://doi.org/10.1101/2021.03.16.435698.

2. Deuterated rhodamines for protein labelling in nanoscopy, Roßmann, K.; Akkaya, K. C.; Charbonnier, C.; Eichhorst, J.; Jones, B.; Lehmann, M.; Broichhagen, J.*bioRxiv 2020, doi: https://doi.org/10.1101/2020.08.17.253880v1.


1. Purification of age-distinct insulin secretory granules through antigen restriction using the CLIP-tag, Neukam, M.; Ganss, K.; Vasiljevic, J.; Broichhagen, J.; Johnsson, K.; Kurth, T.; Solimena, M., bioRxiv 2020, doi: https://doi.org/10.1101/2020.06.03.103770.

 

PUBLICATIONS

48.   Selvakumar, P.; Lee, J.; Khanra, N.; He, C.; Munguba, H.; Kiese, L.; Broichhagen, J.; Reiner, A.; Levitz, J.; Meyerson, J. R., Structural and compositional diversity in the kainate receptor family, Cell Rep. 2021, 37, 109891.

47.   Kemter, E.; Müller, A.; Neukam, M.; Ivanova, A.; Klymiuk, N.; Renner, S.; Yang, K.; Broichhagen, J.; Kurome, M.; Zakhartchenko, V.; Kessler, B.; Knoch, K.-P.; Bickle, M.; Ludwig, B.; Johnsson, K.; Lickert, H.; Kurth, T.; Wolf, E.; Solimena, M., Sequential in vivo labeling of insulin secretory granule pools in INS-SNAP transgenic pigs, Proc. Natl. Acad. Sci. USA 2021118, e2107665118. 


46.   Lucey, M.; Ashik, T.; Marzook, A.; Wang, Y.; Goulding, J.; Oishi, A.; Broichhagen, J.; Hodson, D. J.; Minnion, J.; Elani, Y.; Jockers, R.; Briddon, J. B.; Bloom, S. R.; Tomas, A.; Jones, B., Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking, Mol. Pharmacol. 2021, accepted. 

45.    Olaniru, O. E.; Cheng, J.; Ast, J.; Arvaniti, A.; Atanes, P.; Huang C. G.; King, A. J. F.; Jones, P. M.; Broichhagen, J.; Hodson, D. J.; Persaud, S. J., SNAP-tag-enabled super-resolution imaging reveals constitutive and agonist-dependent trafficking of GPR56 in pancreatic β-cells, Mol. Metab. 2021, 53, 101285.

44.    Marzook, A.; Chen, S.; Pickford, P.; Lucey, M.; Wang, Y.; Corrêa Jr, I.; Broichhagen, J.; Hodson, D. J.; Salem, V.; Rutter, G. A.; Tan, T. M.; Bloom, S. R.; Tomas, A.; Jones, B., Evaluation of efficacy- versus affinity-driven agonism with biased GLP-1R ligands P5 and exendin-F1, Biochem. Pharmacol. 2021, 190, 114656.


43.   Khelashvili, G.; Pillai, A. N.; Lee, J.; Pandey, K.; Payne, A. M.; Siegel, Z.; Cuendet, M. A.; Lewis, T. Y.; Arshavsky, V. Y.; Broichhagen, J.; Levitz, J.; Menon, A. K., Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin, Sci. Rep. 2021, 11, 10536. 

42.   Thibado, J. K.; Tano, J.-Y.; Lee, J.; Salas-Estrada, L.; Provasi, D.; Strauss, A.; Ribeiro, J. M. L.; Xiang, G.; Broichhagen, J.; Filizola, M.; Lohse, M.; Levitz, J., Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors, eLife 2021, 10, e67027.

41.   Gutzeit, V. A.; Acosta-Ruiz, A.; Munguba, H.; Häfner, S., Landra-Willm, A.; Mathes, B.; Mony, J.; Yarotski, D.; Börjesson, K.; Liston, C.; Sandoz, G.; Levitz, J.; Broichhagen, J.*, A fine-tuned azobenzene for enhanced photopharmacology in vivoCell Chem. Bio. 2021, in press, https://doi.org/10.1016/j.chembiol.2021.02.020

40.   Broichhagen, J.#,*; Kilian, N.#,*, Chemical biology tools to investigate malaria parasites, ChemBioChem 2021, 22, 2219–2236. 

39. PhotocagedHoechst enables subnuclear visualization and cell selective staining of DNA in vivo, Lämmle, C. A.; Varady, A.; Müller, T. G.; Sturtzel, C.; Riepl, M.; Mathes, B.; Eichhorst, J.; Sporbert, A.; Lehmann, M.; Kräusslich, H.-G.; Distel, M.; Broichhagen, J.*, ChemBioChem 2021, 22, 548–556. 

38. Ligand-specific factors influencing GLP-1 receptor post-endocytic trafficking and degradation in pancreatic beta cells, Fang, Z.; Chen, S.; Manchanda, Y.; Bitsi, P.; Pickford, P.; David, A.; Shchepinova, M. M.; Corrêa Jr, I.; Hodson, D. J.; Broichhagen, J.; Tate, E. W.; Reimann, F.; Salem, V.; Rutter, G. A.; Tan, T.; Bloom, S. R.; Tomas, A.; Jones, B. J., Int. J. Mol. Sci. 202021, 8404. 

37. Photohormones Enable Optical Control of the Peroxisome Proliferator-Activated Receptor Gamma (PPARg), Hinnah, K.; Willems, S.; Morstein, J.; Heering, J.; Hartrampf, F. W. W.; Broichhagen, J.; Leippe, P.; Merk, D.; Trauner, D., J. Med. Chem. 2020, 63, 10908–10920.

36. Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates, Poc, P.; Gutzeit, V. A.; Ast, J.; Joon, L.; Jones, B. J.; D’Este, E.; Mathes, B.; Lehmann, M.; Hodson, D. J.; Levitz, J.; Broichhagen, J.*, Chem. Sci. 2020, 11, 7871–7883. 

35. Signalling, trafficking and glucoregulatory properties of glucagon-like peptide-1 receptor agonists exendin-4 and lixisenatide, Pickford, P.; Lucey, M.; Fang, Z.; Bitsi, S.; Bernardino de la Serna, J.; Broichhagen, J.; Hodson, D. J.; Minnion, J.; Rutter, G. A.; Bloom, S. R.; Tomas, A.; Jones, B. J., Br. J. Pharmacol. 2020, 117, 7871–7883.

34. The influence of peptide context on signalling and trafficking of glucagon-like peptide-1 receptor biased agonists, Fang, Z.; Chen, S.; Pickford, P.; Broichhagen, J.; Hodson, D. J.; Corrêa Jr, I.; Kumar, S.; Görlitz, F.; Dunsby, C.; French, P.; Rutter, G. A.; Tan, T.; Bloom, S. R.; Tomas, A.; Jones, B. J., ACS Pharmacol. Transl. Sci. 2020, 3, 345–360. 

33. Transformation of Receptor Tyrosine Kinases into Glutamate Receptors and Photoreceptors, Leippe, P.#; Broichhagen, J.#; Cailliau, K.; Mougel, A.; Morel, M.; Dissous, C.; Trauner, D.; Vicogne, J., Angew. Chem. Int. Ed. 2020, 59, 6720–6723.

32. Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics, Ast, J.; Arvaniti, A.; Fine N. H. F.; Nasteska, D.; Ashford, F.; Stamataki, Z.; Koszegi, Z.; Bacon, A.; Jones, B. J.; Lucey, M. A.; Sasaki, S.; Hastoy, B.; Tomas, A.; Reiman, F.; D’Agostino, G.; Reiman, F.; Lynn, F. C.; Reissaus, C.; Linnemann, A. K.; D’Este, E.; Calebiro, D.; Trapp, S.; Johnsson, K.; Podewin, T.*; Broichhagen, J.*; Hodson, D. J.*, Nat. Commun. 202011, 467.

31. Branched photoswitchable tethered ligands enable ultra-efficient optical control and detection of G protein-coupled receptors in vivo, Acosta-Ruiz, A.; Gutzeit, V. A.; Skelly, M. J.; Meadows, S.; Lee, J.; Parekh, P.; Orr, A. G.; Liston, C.; Pleil, K. E.; Broichhagen, J.*; Levitz, J.*, Neuron 2020105, 446–463.

30. Genetically-targeted optical control of an endogenous G protein-coupled receptor, Donthamsetti, P. C.; Broichhagen, J.; Vyklicky, V.; Stanley, C.; Fhu, Z.; Visel, M.; Levitz, J.; Javitch, J. A.; Trauner, D.; Isacoff, E. Y., J. Am. Chem. Soc. 2019141, 11522–11530.

29. CCDC 1884029: Experimental Crystal Structure Determination, Broichhagen, J.; Burg, F.; Häfner, S.; Mayer, P.; Trauner, D., CSD Communication 2018, https://doi.org/10.5517/ccdc.csd.cc217h4y.

28. Optical tools for understanding the complexity of beta cell signaling and insulin release, Frank, J. A.#, Broichhagen, J.#; Yushchenko, D.; Schultz, C.; Trauner, D.; Hodson, D. J., Nat. Rev. Endocrinol. 201814, 721–737.

27. SNAP-Tagged Nanobodies Enable Reversible Optical Control of a G Protein-Coupled Receptor via a Remotely Tethered Photoswitchable Ligand, Farrants, H.; Acosta-Ruiz, A.; Gutzeit, V. A.; Trauner, D.; Johnsson, K.; Levitz, J.*; Broichhagen, J.*, ACS Chem. Bio. 201813, 2682–2688.

26. A New Fluorogenic Small-Molecule Labeling Tool for Surface Diffusion Analysis and Advanced Fluorescence Imaging of β-site Amyloid Precursor Protein-Cleaving Enzyme 1 Based on Silicone Rhodamine: SiR-BACE1, Karch, S.#, Broichhagen, J.#; Schneider, J.#; Böning, D.; Hartmann, S.; Schmid, B.; Tripal, P.; Palmisano, R.; Alzheimer, C.; Johnsson, K.; Huth, T., J. Med. Chem. 201861, 6121–6139.

25. A (+)-larixol congener with high affinity and subtype selectivity towards TRPC6, Häfner, S.; Burg, F.; Kannler, M.; Urban, N.; Mayer, P.; Dietrich, A.; Trauner, D.*; Broichhagen, J.*; Schaefer, M.*, ChemMedChem 201813, 1028–1035.

24. A predictive approach for the optical control of carbonic anhydrase II activity, DuBay, K. H.; Iwan, K.; Osorio-Planes, L.; Geissler, P.; Groll, M.; Trauner, D.; Broichhagen, J.*, ACS Chem. Bio. 201813, 793–800.

23. Conditional and reversible activation of class A and B G protein-coupled receptors using tethered pharmacology, Podewin, T.#; Ast, J.#; Broichhagen, J.#; Fine, N. H. F.; Nasteska, D.; Leippe, P.; Gailer, M.; Buenaventura, T.; Kanda, N.; Jones, B. J.; M’Kadmi, C.; Baneres, J.-L.; Marie, J.; Tomas, A.; Trauner, D.; Hoffmann-Röder, A.; Hodson, D. J., ACS Cent. Sci. 20184, 166–179. 

22. Optical Control of a Biological Reaction-Diffusion System, Glock, P.; Broichhagen, J.; Kretschmer, S., Blumhardt, P.; Müksch, J.; Trauner, D.; Schwille, P., Angew. Chem. Int. Ed. 2018, 57, 2362–2366; Optical Control of a Biological Reaction-Diffusion System, Glock, P.; Broichhagen, J.; Kretschmer, S., Blumhardt, P.; Müksch, J.; Trauner, D.; Schwille, P., Angew. Chem. 2018130, 2386–2390.

21. Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor, Berry, H. M.; Holt, A.; Levitz, J.; Broichhagen, J.; Gaub, B. M.; Visel, M.; Stanley, C.; Kim, Y. J.; Cao, K.; Kramer, R. H.; Trauner, D.; Flannery, J.; Isacoff, E. Y., Nat. Commun. 20178, 1862.

20. Optical control of GPR40 signalling in pancreatic β-cells, Frank, J.; Yushchenko, D.; Fine, N. H. F.; Duca, M.; Citir, M.; Broichhagen, J.; Hodson, D. J.; Schultz, C.; Trauner, D., Chem. Sci. 20178, 7604–7610.

19. Potent Prearranged Positive Allosteric Modulators of the Glucagon-like Peptide-1 Receptor, Jones, B. J.; Scopelliti, R.; Bloom, S. R.; Tomas, A.; Hodson, D. J.*; Broichhagen, J.*, ChemistryOpen 20176, 501–505.

18. Optical control of a receptor-linked guanylyl cyclase using a photoswitchable peptidic hormone, Podewin, T.#; Broichhagen, J.#; Frost, C.; Groneberg, D.; Ast, J.; Meyer-Berg, H.; Fine, N. H. F.; Friebe, A.; Zacharias, M.; Hodson, D. J.; Trauner, D.; Hoffmann-Röder, A., Chem. Sci. 20178, 4644–4653.

17. Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors, Levitz, J.#; Broichhagen, J.#; Leippe, P.; Konrad, D.; Trauner, D.; Isacoff, E. Y., Proc. Natl. Acad. Sci. USA 2017114, E3546–E3554. 

16. Remote control of glucose homeostasis in vivousing photopharmacology, Mehta, Z. B.#; Johnston, N. R.#; Nguyen-Tu, M.-S.#; Broichhagen, J.; Schultz, P.; Larner, D. P.; Leclerc, I.; Trauner, D.; Rutter, G. A.; Hodson, D. J., Sci. Rep. 20177, 291.

15. Beta cell hubs dictate pancreatic islet responses to glucose, Johnston, N. R.; Mitchell, R. K.; Haythorne, E.; Paiva Pessoa, M.-R., Semplici, F.; Ferrer, J.; Piemonti, L.; Marchetti, P.; Bugliani, M.; Bosco, D.; Berishvili, E.; Duncanson, P.; Watkinson, M.; Broichhagen, J.; Trauner, D.; Rutter, G. A.; Hodson, D. J., Cell Metab. 201624, 1–13.

14. Allosteric Optical Control of a Class B G-Protein-Coupled Receptor, Broichhagen, J.; von Ohlen, Y.; Johnston, N. R.; Meyer-Berg, H.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Trauner, D.; Hodson, D. J., Angew. Chem. Int. Ed. 201655, 5865–5868; Allosterische optische Steuerung eines Klasse-B-G-Protein-gekoppelten Rezeptors, Broichhagen, J.; von Ohlen, Y.; Johnston, N. R.; Meyer-Berg, H.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Trauner, D.; Hodson, D. J., Angew. Chem. 2016128, 5961–5965.

13. 6-(6-(Pyridin-2-yl)-1,2,4,5-tetrazin-3-yl)pyridin-3-amine monohydrate, Broichhagen, J.; Klingl, Y. E.; Trauner, D.; Mayer, P., Acta Cryst. 2016E72, 238–240.

12. Optical Control of Insulin Release Using an Incretin Switch, Broichhagen, J.#; Podewin, T.#; Meyer-Berg, H.; von Ohlen, Y.; Johnston, N. R.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Hoffmann-Röder, A.; Hodson, D. J.; Trauner, D., Angew. Chem. Int. Ed. 201554, 15565–15569; Optische Kontrolle der Insulinsekretion mit einem Inkretinschalter, Broichhagen, J.#; Podewin, T.#; Meyer-Berg, H.; von Ohlen, Y.; Johnston, N. R.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Hoffmann-Röder, A.; Hodson, D. J.; Trauner, D., Angew. Chem. 2015127, 15786–15790.

11. Orthogonal optical control of a G protein-coupled receptor with a SNAP-tethered photochromic ligand, Broichhagen, J.#; Damijonaitis, A.#; Levitz, J.#; Sokol, K. R.; Leippe, P.; Konrad, D.; Isacoff, E. Y.; Trauner, D., ACS Cent. Sci. 20151, 383–393. 

10. A roadmap to success in photopharmacology, Broichhagen, J.; Frank, J. A.; Trauner D., Acc. Chem. Res. 201548, 1947–1960.

9.   Azobenzene-based inhibitors of human carbonic anhydrase II, Runtsch, L. S.; Barber, D. M.; Mayer, P.; Groll, M.; Trauner, D.*; Broichhagen, J.*, Beilstein J. Org. Chem. 201511, 1129–1135.

8.   Crystal structure of (E)-1,2-bis(4-bromo-2,6-difluorophenyl)diazene, Broichhagen, J.; Woodmansee, D. H.; Trauner, D.; Mayer, P., Acta Cryst. 2015, E71, o459–o460.

7.   A red-shifted photochromic sulfonylurea for the remote control of pancreatic beta cell function, Broichhagen, J.; Frank, J. A.; Johnston, N. R.; Mitchell, R. K.; Smid, K.; Marchetti, P.; Bugliani, M.; Rutter, G. A.; Trauner, D.; Hodson, D. J., Chem. Commun. 201551, 6018–6021. 

6.   AzoCholine enables optical control of alpha 7 nicotinic acetylcholine receptors in neural networks,Damijonaitis, A.; Broichhagen, J.; Hüll, K.; Nagpal, J.; Laprell, L.; Woodmansee, D. H.; Urushima, T.; Schönberger, M.; Rafiq, A.; Sumser, M. P.; Kummer, W.; Gottschalk, A.; Trauner, D., ACS Chem. Neurosci. 20155, 701–707.

5.   The in vivo chemistry of photochromic tethered ligands, Broichhagen, J.; Trauner, D., Curr. Opin. Chem. Biol. 201421, 121–127.

4.   Optical control of insulin release using a photoswitchable sulfonylurea, Broichhagen, J.; Schönberger, M.; Cork, S. C.; Frank, J. A.; Marchetti, P.; Bugliani, A. M.; Shapiro, A. M. J.; Trapp, S.; Rutter, G. A.; Hodson, D. J.; Trauner, D., Nat. Commun. 20145, 5116.

3.   Optical Control of Acetylcholinesterase with a Tacrine Switch, Broichhagen, J.; Jurastow, I.; Iwan, K.; Kummer, W.; Trauner, D., Angew. Chem. Int. Ed. 201453, 7657-7660; Optische Kontrolle der Acetylcholinesterase mit einem schaltbaren Tacrin, Broichhagen, J.; Jurastow, I.; Iwan, K.; Kummer, W.; Trauner, D., Angew. Chem. 2014126, 7788–7792.

2.   Bilirubin in a New Light, Broichhagen, J.; Trauner, D., Angew. Chem. Int. Ed. 201352, 13868-13870; Bilirubin in einem neuen Licht, Broichhagen, J.; Trauner, D., Angew. Chem. 2013125, 14112–14114.

1.   Strain-Promoted Alkyne Azide Cycloaddition for the Functionalization of Poly(amide)-Based Dendrons and Dendrimers, Ornelas, C.; Broichhagen, J.; Weck, M., J. Am. Chem. Soc. 2010132, 3923–3931.

# these authors contributed equally

* corresponding author

 

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ALUMNI

CORENTIN CHARBONNIER

Research intern
June – September 2020

NINA DIEDERICH

Research intern

November 2020 – January 2021

CHRISTIANE HUHN

Research intern
November 2020 – March 2021

ABHA VALAVALKAR

Research Intern
January – March 2021

TRISTAN REIF

HiWi
January – April 2021

PHILIP SLAWETZKI

Intern
April – July 2021

JESSICA ILLE

Intern
May – July 2021

MICHAEL TRUMPP

Intern
May – August 2021

DENISE HEIDLER

Intern
August 2021

 

CONTACT

Leibniz-Forschungsinstitut für

Molekulare Pharmakologie (FMP)

Robert-Roessle-Str. 10

13125 Berlin

Germany

+49–(0)30–94793–171

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