JB LAB
Dr. Johannes Broichhagen
Junior Group Leader
Leibniz-FMP Berlin
RESEARCH
MEMBERS
JOHANNES BROICHHAGEN
Group Leader
JB studied chemistry at the University of Erlangen-Nuremberg including a one-year stint with Marcus Weck at NYU and graduated in 2010 with honors. He then joined the group of Dirk Trauner at LMU Munich to dive into Chemical Biology and obtained his PhD in 2014. Next, he was working with Kai Johnsson at EPF Lausanne and at the MPI for Medical Research in Heidelberg. Since March 2020, he joined the FMP in Berlin as a Junior Group Leader.
RAMONA BIRKE
Technical Assistant
Ramona studied Biology with a focus on genetics and molecular biology at the Humboldt University Berlin, graduating 2010 in Molecular Ecology. After a stint at WITA GmbH, she joined the FMP as technical assistant in 2011, working in the field Molecular Cellular Physiology and Mass Spectrometry. Enjoying nature and being a passionate hiker in her free time, she teamed up with JB in 2020 to support and pursue our quests in Chemical Biology.
KILIAN ROSSMANN
PhD Student
Kilian studied chemistry at the Julius-Maximilian-University Würzburg (JMU) with focus on Organic, Physical and Medicinal Chemistry. For his Master Thesis he joined the group of Dirk Trauner at New York University (NYU), where he worked on photoswitchable PROTACs. After graduating in 2019, Kilian decided to join JB’s lab in 2020 to work on novel imaging techniques.
SOUVIK GHOSH
PostDoc
Souvik completed his Integrated M.Sc in Chemistry from S.V. National Institute of Technology, India. Then, he move to Bremen, Germany to pursue his PhD in 2018. He designed and synthesized a series of photoswitchable azobenzene macrocycles and investigated their photophysical properties during his PhD. Afterwards, he joined JB's lab, focusing on the development of deuterated compounds for sensitive microscopy.
LAURA KNELLER
PostDoc
Laura studied Toxicology at Charité – Universitätsmedizin Berlin and did her Master's research with Joëlle Rüegg at the Karolinska Institute in Stockholm. She then returned to Berlin for her PhD in Infectiology in the group of Martin Witzenrath, focusing on pneumonia-induced lung barrier failure. In 2024, she joined JB's lab to work on beta cell replacement and protection in type 1 diabetes.
BLAISE GATIN-FRAUDET
PostDoc
Blaise studied chemistry at the University of Paris-Saclay and at the ENS Paris-Saclay. He obtained his PhD in 2020 in Chemical Biology under the supervision of Dominique Urban (ICMMO) and Boris Vauzeilles (ICSN). Then, he worked with Pierre-Yves Renard and Ludovic Jean at COBRA in Rouen. In 2023, he moved to Berlin to join Sigrid Milles' group and JB's lab to work on innovative labeling strategies for high resolution spectroscopic techniques.
ABIGAIL LEWIS
PhD Student
Abigail studied Chemistry at Northwestern University after which she worked on various research projects at Argonne National Laboratory and AbbVie Inc. She then moved to Amsterdam to obtain her M.Sc. in Drug Design and Synthesis. In 2023 she joined the FMP as a PhD student in Fan Liu’s lab developing novel crosslinkers for investigating structural interactomics via mass spectrometry, collaboratively hosted in our lab for synthetic work.
SONG CHEN
PhD Student
Song studied organic chemistry and obtained his M.Sc. under the supervision of Prof. Bin Tan and Prof. Shaoyu Li at Southern University of Science and Technology (SUSTech) in China. He then developed an interest in chemical biology, which led him to spend two years in Lausanne. In October 2024, he started his Ph.D. at FMP with JB to investigate deuterated photoswitched probes.
SYLVESTRE BACHOLLET
PostDoc
Sylvestre studied chemistry at the ENSC inMontpellier and obtained his PhD in 2016 in Organic Chemistry (Marie Curie ITN grant) in the group of Joseph Harrity at U Sheffield, UK, including a six-month secondment with Stefan Bräse at the KIT in Germany. He joined the group of Stephen Hanessian at U Montréal, worked at the ENS Paris, with Blaise Dumat, Laurence Grimaud and Maxime Vitale, before looking into bioconjugation with Patricia Busca at Université Paris Cité . In 2024, he joined the groups of JB and Daniel Roderer at FMP to work on the development of Cryo-CLEM probes supported by CZI.
CHRISTINA HOLMBOE OLESEN
PostDoc
Christina completed her studies in biochemistry at University of Copenhagen, Denmark, where she earned her PhD in 2023 under the supervision of Jannick Prentø and Jens Bukh. During her PhD, she focused on investigating the interaction between hepatitis C virus (HCV) envelope proteins and neutralizing antibodies. Through collaborative efforts within her research group, Christina successfully determined the structure of the HCV envelope protein complex. In 2024, she transitioned to a new role within the research group led by JB, where she is working on developing of a minimal tag for protein labeling and imaging applications.
Photos by Silke Oßwald or Matthias Rethmann.
OPEN POSITIONS
Check here:
PUBLICATIONS
77. Roßmann, K.; Gonzalez-Hernandez, A. J.; Bhujan, K.; Schattenberg, C.; Sun, H.; Börjesson, K.; Levitz, J.*; Broichhagen, J.*, Deuteration as a general strategy to enhance azobenzene-based photopharmacology, Angewandte Chemie International Edition 2024, doi: 10.1002/anie.202408300.
76. Montaner, M.; Denom, J.; Simon, V.; Jiang, W.; Holt, M. K.; Brierley, D.; Rouch, C.; Foppen, E.; Nadim, K.; Jarriault, D.; Khan, D.; Eygret, L.; Mifsud, F.; Hodson D. J.; Broichhagen, J.; Van Oudenhove, L.; Fioramonti, X.; Gault, V. A.; Cota, D.; Reimann, F.; Gribble, F. M.; Migrenne-Li, S.; Trapp, S.; Gurden, H.; Magnan, C., A neuronal circuit driven by GLP-1 in the olfactory bulb regulates insulin secretion, Nat. Commun. 2024, 15, 6941.
75. Stockinger, F.; Poc, P.; Möhwald, A.; Karch, S.; Häfner, S.; Alzheimer, C.; Sandoz, G.; Huth, T.*; Broichhagen, J.*, Multi-color, cell-impermeable and high affinity BACE1 inhibitor probes enable superior endogenous staining and imaging of single molecules, J. Med. Chem. 2024, 67, 10152–10167.
74. Shilleh, A. H.; Viloria, K.; Broichhagen, J.; Campbell, J. E.; Hodson, D. J., GLP1R and GIPR expression and signaling in pancreatic alpha cells, beta cells and delta cells, Peptides 2024, accepted.
73. Neukam, M.; Sala, P.; Brunner, A.-D.; Ganß, K.; Palladini, A.; Grzybek, M.; Topcheva, O.; Vasiljević, J.; Broichhagen, J.; Johnsson, K.; Kurth, T.; Mann, M.; Coskun, Ü.; Solimena, M., Purification of time-resolved insulin granules reveals proteomic and lipidomic changes during granule aging, Cell Rep. 2024, 43, 113836.
72. Lee, J.; Gonzalez-Hernandez, A. J.; Kristt, M.; Abreu, N.; Roßmann, K.; Arefin, A.; Marx, D.; Broichhagen, J.; Levitz, J., Distinct beta-arrestin coupling and intracellular trafficking of metabotropic glutamate receptor homo- and hetero-dimers, Sci. Adv. 2023, 9, eadi8076.
71. Rückert, A.-K.; Ast, J.; Hasib, A.; Nasteska, D.; Viloria, K.; Broichhagen, J.*; Hodson, D. J.*, Fine-tuned photochromic sulfonylureas for the optical control of beta cell Ca2+ fluxes, Diabetic Medicine 2023, 40, e15220.
70. Lightley, J.; Kumar, S.; Lim, M. Q.; Garcia, E.; Görlitz, F.; Alexandrov, Y.; Parrado, T.; Hollick, C.; Steele, E.; Roßmann, K.; Graham, J.; Broichhagen, J.; McNeish, I.; Roufosse, C. A.; Neil, M. A. A.; Dunsby, C.; French, P. M. W., openFrame: a modular, sustainable, open microscopy platform with single-shot, dual-axis optical autofocus module providing high precision and long range of operation, J. Microsc. 2023, 292, 64–77.
69. Ruwolt, M.; He, Y.; Borges Lima, D.; Barshop, W.; Broichhagen, J.; Huguet, R.; Viner, R.; Liu, F., Real-time library search assisted identification of cross-links increases resolution for structural modeling and interactome mapping, Anal. Chem. 2023, 95, 5248–5255.
68. Velicky, P.; Miguel, E.; Michalska, J. M.; Wei, D.; Lin, Z.; Watson, J. F.; Troidl, J.; Beyer, J.; Ben-Simon, Y.; Sommer, C.; Jahr, W.; Cenameri, A.; Broichhagen, J.; Grant, S. G. N.; Jonas, P.; Novarino, G.; Pfister, H.; Bickel, B.; Danzl, J. G., Dense 4D nanoscale reconstructon of living brain tissue, Nat. Meth. 2023, 20, 1256–1265.
67. Adriaenssens, A.; Broichhagen, J.; De Bray, A.; Ast, J.; Hasib, A.; Jones, B.; Tomas, A.; Burgos, N. F.; Woodward, O..; Lewis, J.; O’Flaherty, E.; El, K.; Cui, C.; Harada, N.; Inagaki, N.; Campbell, J.; Brierley, D.; Hodson, D. J.; Samms, R.; Gribble, F.; Reimann, F., Glucose-dependent insulinotropic polypeptide receptor (GIPR)-expressing neurons in the hypothalamus and the dorsal vagal complex employ distinct mechanisms to affect feeding behaviour, JCI Insight 2023, 8, e164921.
66. Manchanda, Y.; Bitsi, S.; Chen, S.; Broichhagen, J.; de la Serna, J. B.; Jones, B.; Tomas, A., Enhanced endosomal signaling and desensitization of GLP-1R versus GIPR in pancreatic beta cells, Endocrinology 2023, doi: 10.1210/endocr/bqad028.
65. Jain, A.; Broichhagen, J.*, Make it stick: fixable ligands for tissue imaging, Chem 2023, 9, 260–262.
64. Ast, J.; Nasteska, D.; Fine N. H. F.; Nieves, D.; Koszegi, Z.; Lanoiselée, Y.; Cuozzo, F.; Viloria, K.; Bacon, A.; Luu, N.-T.; Newsome, P. N.; Calebiro, D.; Owen, D.; Broichhagen, J.*; Hodson, D. J.*, Revealing the tissue-level complexity of endogenous glucagon-like peptide 1-receptor expression and signaling, Nat. Commun. 2023, 14, 301.
63. Jatzlau, J.; Burdzinski, W.; S.; Trumpp, M.; Obendorf, L.; Roßmann, K.; Ravn, K.; Hyvönen, M.; Bottanelli, F.; Broichhagen, J.; Knaus, P., A versatile Halo- and SNAP-tagged BMP/TGFβ receptor library for quantification of cell surface ligand binding, Commun. Biol. 2023, 6, 34.
62. Trumpp, M.; Oliveras, A.; Gonschior, H.; Ast, J.; Hodson, D. J.; Knaus, P.; Lehmann, M.; Birol, M.*; Broichhagen, J.*, Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy, Chem. Commun. 2022, 58, 13724–13727.
61. Xiang, G.; Acosta-Ruiz, A.; Radoux-Mergault, A.; Kristt, M.; Kim, J.; Moon, J. D.; Broichhagen, J.; Inoue, A.; Lee, F. S.; Stoeber, M.; Dittman, J. S.; Levitz, J., Control of Gaq signaling dynamics and GPCR cross-talk by GRKs, Sci. Adv. 2022, 8, eabq3363.
60. Stieger, C. E.; Park, YT.; de Geus, M. A. R.; Kim, D.; Huhn, C.; Slenczka, J. S.; Ochtrop, P.; Müchler, J. M.; Süssmuth, R.; Broichhagen, J.; Baik, M.-H.; Hackenberger, C. P. R., DFT-guided Discovery of Ethynyl-Triazolyl-Phosphinates for Chemoselective Cysteine Bioconjugation and Profiling, Angew. Chem. Int. Ed. 2022, 61, e202205348..
59. Roßmann, K.; Akkaya, K. C.; Poc, P.; Charbonnier, C.; Eichhorst, J.; Gonschior, H.; Valavalkar, A.; Wendler, N.; Cordes, T.; Dietzek-Ivanšić, B.; Jones, B.; Lehmann, M.; Broichhagen, J.*, N-Methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy, Chem. Sci. 2022, 13, 8605–8617.
58. Berry, M.; Holt, A.; Broichhagen, J.; Donthamsetti, P.; Flannery, J. G.; Isacoff, E. Y., Photopharmacology for vision restoration, Curr. Opin. Pharmacol. 2022, 65, 102259.
57. Huhn, C.; Broichhagen, J.*, Visualisierung endogener Proteine in ihrer natürlichen Umgebung, Biospektrum (Heidelb.) 2022, 3, 264–268.
56. Cahill, K. N.; Amin, T.; Boutaud, O.; Printz, R.; Newcomb, D.; Hodson, D. J.; Broichhagen, J.; Beckman, J.; Mashayekhi, M.; Luther, M.; Brown, N.; Peebles, R. S.; Niswender, K., Glucagon-like peptide-1 receptor regulates thromboxane-induced human platelet activation, JACC: Basic Transl. Sci. 2022, accepted.
55. Ast, J.; Novak, A. N.; Podewin, P.; Fine, N. H. F.; Jones, B.; Tomas, A.; Birke, R.; Roßmann, K.; Mathes, B.; Eichhorst, J.; Lehmann, M.; Linnemann, A. K.*; Hodson, D. J*.; Broichhagen, J.*, An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo, JACS Au 2022, 2, 1007–1017.
54. Mugabo, Y.; Zhao, C.; Tan, J. J.; Ghosh, A.; Campbell, S. A.; Fadzeyeva, E.; Paré, F.; Pan, S. S.; Galipeau, M.; Ast, J.; Broichhagen, J.; Hodson, D. J.; Mulvihill, E. E.; Petropoulos, S.; Lim, G. E., 14-3-3ζ constrains insulin secretion by regulating mitochondrial function in pancreatic β-cells, JCI Insight 2022, 7, e156378. .
53. Broichhagen, J.*; Levitz, J.*, Advances in tethered photopharmacology for precise optical control of signaling proteins, Curr. Opin. Pharmacol. 2022, 63, 102196.
52. Gazzi, T.; Brennecke, B.; Atz, K.; Korn, C.; Sykes, D.; Forn-Cuni, G.; Pfaff, P.; Sarott, R. C.; Westphal, M. V.; Mostinski, Y.; Mach, L.; Wasinska-Kalwa, M.; Weise, M.; Hoare, B. L.; Miljuš, T.; Mexi, M.; Roth, N.; Koers, E. J.; Guba, W.; Alker, A.; Rufer, A. C.; Kusznir, E. A.; Huber, S.; Raposo, C.; Zirwes, E. A.; Osterwald, A.; Pavlovic, A.; Moes, S.; Beck, J.; Nettekoven, M.; Benito-Cuesta, I.; Grande, T.; Drawnel, F.; Widmer, G.; Holzer, D.; van der Wel, T.; Mandhair, H.; Grimsey, N.; Honer, M.; Fingerle, J.; Scheffel, J.; Broichhagen, J.; Gawrisch, K.; Romero, J.; Hillard, C. J.; Varga, Z. V. van der Stelt, M.; Pacher, P.; Gertsch, J.; Ullmer, J.; McCormick, P. J.; Oddi, S.; Spaink, H. P.; Maccarrone, M.; Veprintsev, D. B.; Carreira, E. M.; Grether, U.; Nazaré, M., Detection of Cannabinoid Receptor Type 2 in Native Cells and Zebrafish with a Highly Potent, Cell-Permeable Fluorescent Probe, Chem. Sci. 2022, 13, 5539–5545.
51. Pauza, A. G., Thakkar, P.; Tasic, T.; Felippe, I.; Bishop, P.; Greenwood, M. P.; Rysevaite-Kyguoliene, K.; Ast, J.; Broichhagen, J.; Hodson, D. J.; Salgado, H. C.; Pauza, D. H.; Japundzic-Zigon, N.; Paton, J. F. R.; Murphy, D., GLP1R attenuates sympathetic response to high glucose via carotid body inhibition, Circ. Res. 2022, 130, 694–707.
50. Birke, R.; Ast, J.; Roosen, D. A.; Lee, J.; Roßmann, K.; Huhn, C.; Mathes, B.; Lisurek, M.; Bushiri, D.; Sun, H.; Jones, B.; Lehmann, M.; Levitz, J.; Haucke, V.; Hodson, D. J.; Broichhagen, J.*, Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins, Org. Biomol. Chem. 2022, doi: 10.1039/D1OB02216D.
49. Ast, J.; Broichhagen, J.*; Hodson, D. J.*, Reagents and models for detecting endogenous GLP1R and GIPR, EBioMedicine 2021, 74, 103739.
48. Selvakumar, P.; Lee, J.; Khanra, N.; He, C.; Munguba, H.; Kiese, L.; Broichhagen, J.; Reiner, A.; Levitz, J.; Meyerson, J. R., Structural and compositional diversity in the kainate receptor family, Cell Rep. 2021, 37, 109891.
47. Kemter, E.; Müller, A.; Neukam, M.; Ivanova, A.; Klymiuk, N.; Renner, S.; Yang, K.; Broichhagen, J.; Kurome, M.; Zakhartchenko, V.; Kessler, B.; Knoch, K.-P.; Bickle, M.; Ludwig, B.; Johnsson, K.; Lickert, H.; Kurth, T.; Wolf, E.; Solimena, M., Sequential in vivo labeling of insulin secretory granule pools in INS-SNAP transgenic pigs, Proc. Natl. Acad. Sci. USA 2021, 118, e2107665118.
46. Lucey, M.; Ashik, T.; Marzook, A.; Wang, Y.; Goulding, J.; Oishi, A.; Broichhagen, J.; Hodson, D. J.; Minnion, J.; Elani, Y.; Jockers, R.; Briddon, J. B.; Bloom, S. R.; Tomas, A.; Jones, B., Acylation of the incretin peptide exendin-4 directly impacts GLP-1 receptor signalling and trafficking, Mol. Pharmacol. 2021, 100, 319–334.
45. Olaniru, O. E.; Cheng, J.; Ast, J.; Arvaniti, A.; Atanes, P.; Huang C. G.; King, A. J. F.; Jones, P. M.; Broichhagen, J.; Hodson, D. J.; Persaud, S. J., SNAP-tag-enabled super-resolution imaging reveals constitutive and agonist-dependent trafficking of GPR56 in pancreatic β-cells, Mol. Metab. 2021, 53, 101285.
44. Marzook, A.; Chen, S.; Pickford, P.; Lucey, M.; Wang, Y.; Corrêa Jr, I.; Broichhagen, J.; Hodson, D. J.; Salem, V.; Rutter, G. A.; Tan, T. M.; Bloom, S. R.; Tomas, A.; Jones, B., Evaluation of efficacy- versus affinity-driven agonism with biased GLP-1R ligands P5 and exendin-F1, Biochem. Pharmacol. 2021, 190, 114656.
43. Khelashvili, G.; Pillai, A. N.; Lee, J.; Pandey, K.; Payne, A. M.; Siegel, Z.; Cuendet, M. A.; Lewis, T. Y.; Arshavsky, V. Y.; Broichhagen, J.; Levitz, J.; Menon, A. K., Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin, Sci. Rep. 2021, 11, 10536.
42. Thibado, J. K.; Tano, J.-Y.; Lee, J.; Salas-Estrada, L.; Provasi, D.; Strauss, A.; Ribeiro, J. M. L.; Xiang, G.; Broichhagen, J.; Filizola, M.; Lohse, M.; Levitz, J., Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors, eLife 2021, 10, e67027.
41. Gutzeit, V. A.; Acosta-Ruiz, A.; Munguba, H.; Häfner, S., Landra-Willm, A.; Mathes, B.; Mony, J.; Yarotski, D.; Börjesson, K.; Liston, C.; Sandoz, G.; Levitz, J.; Broichhagen, J.*, A fine-tuned azobenzene for enhanced photopharmacology in vivo, Cell Chem. Bio. 2021, in press, https://doi.org/10.1016/j.chembiol.2021.02.020
40. Broichhagen, J.#,*; Kilian, N.#,*, Chemical biology tools to investigate malaria parasites, ChemBioChem 2021, 22, 2219–2236.
39. PhotocagedHoechst enables subnuclear visualization and cell selective staining of DNA in vivo, Lämmle, C. A.; Varady, A.; Müller, T. G.; Sturtzel, C.; Riepl, M.; Mathes, B.; Eichhorst, J.; Sporbert, A.; Lehmann, M.; Kräusslich, H.-G.; Distel, M.; Broichhagen, J.*, ChemBioChem 2021, 22, 548–556.
38. Ligand-specific factors influencing GLP-1 receptor post-endocytic trafficking and degradation in pancreatic beta cells, Fang, Z.; Chen, S.; Manchanda, Y.; Bitsi, P.; Pickford, P.; David, A.; Shchepinova, M. M.; Corrêa Jr, I.; Hodson, D. J.; Broichhagen, J.; Tate, E. W.; Reimann, F.; Salem, V.; Rutter, G. A.; Tan, T.; Bloom, S. R.; Tomas, A.; Jones, B. J., Int. J. Mol. Sci. 2020, 21, 8404.
37. Photohormones Enable Optical Control of the Peroxisome Proliferator-Activated Receptor Gamma (PPARg), Hinnah, K.; Willems, S.; Morstein, J.; Heering, J.; Hartrampf, F. W. W.; Broichhagen, J.; Leippe, P.; Merk, D.; Trauner, D., J. Med. Chem. 2020, 63, 10908–10920.
36. Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates, Poc, P.; Gutzeit, V. A.; Ast, J.; Joon, L.; Jones, B. J.; D’Este, E.; Mathes, B.; Lehmann, M.; Hodson, D. J.; Levitz, J.; Broichhagen, J.*, Chem. Sci. 2020, 11, 7871–7883.
35. Signalling, trafficking and glucoregulatory properties of glucagon-like peptide-1 receptor agonists exendin-4 and lixisenatide, Pickford, P.; Lucey, M.; Fang, Z.; Bitsi, S.; Bernardino de la Serna, J.; Broichhagen, J.; Hodson, D. J.; Minnion, J.; Rutter, G. A.; Bloom, S. R.; Tomas, A.; Jones, B. J., Br. J. Pharmacol. 2020, 117, 7871–7883.
34. The influence of peptide context on signalling and trafficking of glucagon-like peptide-1 receptor biased agonists, Fang, Z.; Chen, S.; Pickford, P.; Broichhagen, J.; Hodson, D. J.; Corrêa Jr, I.; Kumar, S.; Görlitz, F.; Dunsby, C.; French, P.; Rutter, G. A.; Tan, T.; Bloom, S. R.; Tomas, A.; Jones, B. J., ACS Pharmacol. Transl. Sci. 2020, 3, 345–360.
33. Transformation of Receptor Tyrosine Kinases into Glutamate Receptors and Photoreceptors, Leippe, P.#; Broichhagen, J.#; Cailliau, K.; Mougel, A.; Morel, M.; Dissous, C.; Trauner, D.; Vicogne, J., Angew. Chem. Int. Ed. 2020, 59, 6720–6723.
32. Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics, Ast, J.; Arvaniti, A.; Fine N. H. F.; Nasteska, D.; Ashford, F.; Stamataki, Z.; Koszegi, Z.; Bacon, A.; Jones, B. J.; Lucey, M. A.; Sasaki, S.; Hastoy, B.; Tomas, A.; Reiman, F.; D’Agostino, G.; Reiman, F.; Lynn, F. C.; Reissaus, C.; Linnemann, A. K.; D’Este, E.; Calebiro, D.; Trapp, S.; Johnsson, K.; Podewin, T.*; Broichhagen, J.*; Hodson, D. J.*, Nat. Commun. 2020, 11, 467.
31. Branched photoswitchable tethered ligands enable ultra-efficient optical control and detection of G protein-coupled receptors in vivo, Acosta-Ruiz, A.; Gutzeit, V. A.; Skelly, M. J.; Meadows, S.; Lee, J.; Parekh, P.; Orr, A. G.; Liston, C.; Pleil, K. E.; Broichhagen, J.*; Levitz, J.*, Neuron 2020, 105, 446–463.
30. Genetically-targeted optical control of an endogenous G protein-coupled receptor, Donthamsetti, P. C.; Broichhagen, J.; Vyklicky, V.; Stanley, C.; Fhu, Z.; Visel, M.; Levitz, J.; Javitch, J. A.; Trauner, D.; Isacoff, E. Y., J. Am. Chem. Soc. 2019, 141, 11522–11530.
29. CCDC 1884029: Experimental Crystal Structure Determination, Broichhagen, J.; Burg, F.; Häfner, S.; Mayer, P.; Trauner, D., CSD Communication 2018, https://doi.org/10.5517/ccdc.csd.cc217h4y.
28. Optical tools for understanding the complexity of beta cell signaling and insulin release, Frank, J. A.#, Broichhagen, J.#; Yushchenko, D.; Schultz, C.; Trauner, D.; Hodson, D. J., Nat. Rev. Endocrinol. 2018, 14, 721–737.
27. SNAP-Tagged Nanobodies Enable Reversible Optical Control of a G Protein-Coupled Receptor via a Remotely Tethered Photoswitchable Ligand, Farrants, H.; Acosta-Ruiz, A.; Gutzeit, V. A.; Trauner, D.; Johnsson, K.; Levitz, J.*; Broichhagen, J.*, ACS Chem. Bio. 2018, 13, 2682–2688.
26. A New Fluorogenic Small-Molecule Labeling Tool for Surface Diffusion Analysis and Advanced Fluorescence Imaging of β-site Amyloid Precursor Protein-Cleaving Enzyme 1 Based on Silicone Rhodamine: SiR-BACE1, Karch, S.#, Broichhagen, J.#; Schneider, J.#; Böning, D.; Hartmann, S.; Schmid, B.; Tripal, P.; Palmisano, R.; Alzheimer, C.; Johnsson, K.; Huth, T., J. Med. Chem. 2018, 61, 6121–6139.
25. A (+)-larixol congener with high affinity and subtype selectivity towards TRPC6, Häfner, S.; Burg, F.; Kannler, M.; Urban, N.; Mayer, P.; Dietrich, A.; Trauner, D.*; Broichhagen, J.*; Schaefer, M.*, ChemMedChem 2018, 13, 1028–1035.
24. A predictive approach for the optical control of carbonic anhydrase II activity, DuBay, K. H.; Iwan, K.; Osorio-Planes, L.; Geissler, P.; Groll, M.; Trauner, D.; Broichhagen, J.*, ACS Chem. Bio. 2018, 13, 793–800.
23. Conditional and reversible activation of class A and B G protein-coupled receptors using tethered pharmacology, Podewin, T.#; Ast, J.#; Broichhagen, J.#; Fine, N. H. F.; Nasteska, D.; Leippe, P.; Gailer, M.; Buenaventura, T.; Kanda, N.; Jones, B. J.; M’Kadmi, C.; Baneres, J.-L.; Marie, J.; Tomas, A.; Trauner, D.; Hoffmann-Röder, A.; Hodson, D. J., ACS Cent. Sci. 2018, 4, 166–179.
22. Optical Control of a Biological Reaction-Diffusion System, Glock, P.; Broichhagen, J.; Kretschmer, S., Blumhardt, P.; Müksch, J.; Trauner, D.; Schwille, P., Angew. Chem. Int. Ed. 2018, 57, 2362–2366; Optical Control of a Biological Reaction-Diffusion System, Glock, P.; Broichhagen, J.; Kretschmer, S., Blumhardt, P.; Müksch, J.; Trauner, D.; Schwille, P., Angew. Chem. 2018, 130, 2386–2390.
21. Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor, Berry, H. M.; Holt, A.; Levitz, J.; Broichhagen, J.; Gaub, B. M.; Visel, M.; Stanley, C.; Kim, Y. J.; Cao, K.; Kramer, R. H.; Trauner, D.; Flannery, J.; Isacoff, E. Y., Nat. Commun. 2017, 8, 1862.
20. Optical control of GPR40 signalling in pancreatic β-cells, Frank, J.; Yushchenko, D.; Fine, N. H. F.; Duca, M.; Citir, M.; Broichhagen, J.; Hodson, D. J.; Schultz, C.; Trauner, D., Chem. Sci. 2017, 8, 7604–7610.
19. Potent Prearranged Positive Allosteric Modulators of the Glucagon-like Peptide-1 Receptor, Jones, B. J.; Scopelliti, R.; Bloom, S. R.; Tomas, A.; Hodson, D. J.*; Broichhagen, J.*, ChemistryOpen 2017, 6, 501–505.
18. Optical control of a receptor-linked guanylyl cyclase using a photoswitchable peptidic hormone, Podewin, T.#; Broichhagen, J.#; Frost, C.; Groneberg, D.; Ast, J.; Meyer-Berg, H.; Fine, N. H. F.; Friebe, A.; Zacharias, M.; Hodson, D. J.; Trauner, D.; Hoffmann-Röder, A., Chem. Sci. 2017, 8, 4644–4653.
17. Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors, Levitz, J.#; Broichhagen, J.#; Leippe, P.; Konrad, D.; Trauner, D.; Isacoff, E. Y., Proc. Natl. Acad. Sci. USA 2017, 114, E3546–E3554.
16. Remote control of glucose homeostasis in vivousing photopharmacology, Mehta, Z. B.#; Johnston, N. R.#; Nguyen-Tu, M.-S.#; Broichhagen, J.; Schultz, P.; Larner, D. P.; Leclerc, I.; Trauner, D.; Rutter, G. A.; Hodson, D. J., Sci. Rep. 2017, 7, 291.
15. Beta cell hubs dictate pancreatic islet responses to glucose, Johnston, N. R.; Mitchell, R. K.; Haythorne, E.; Paiva Pessoa, M.-R., Semplici, F.; Ferrer, J.; Piemonti, L.; Marchetti, P.; Bugliani, M.; Bosco, D.; Berishvili, E.; Duncanson, P.; Watkinson, M.; Broichhagen, J.; Trauner, D.; Rutter, G. A.; Hodson, D. J., Cell Metab. 2016, 24, 1–13.
14. Allosteric Optical Control of a Class B G-Protein-Coupled Receptor, Broichhagen, J.; von Ohlen, Y.; Johnston, N. R.; Meyer-Berg, H.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Trauner, D.; Hodson, D. J., Angew. Chem. Int. Ed. 2016, 55, 5865–5868; Allosterische optische Steuerung eines Klasse-B-G-Protein-gekoppelten Rezeptors, Broichhagen, J.; von Ohlen, Y.; Johnston, N. R.; Meyer-Berg, H.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Trauner, D.; Hodson, D. J., Angew. Chem. 2016, 128, 5961–5965.
13. 6-(6-(Pyridin-2-yl)-1,2,4,5-tetrazin-3-yl)pyridin-3-amine monohydrate, Broichhagen, J.; Klingl, Y. E.; Trauner, D.; Mayer, P., Acta Cryst. 2016, E72, 238–240.
12. Optical Control of Insulin Release Using an Incretin Switch, Broichhagen, J.#; Podewin, T.#; Meyer-Berg, H.; von Ohlen, Y.; Johnston, N. R.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Hoffmann-Röder, A.; Hodson, D. J.; Trauner, D., Angew. Chem. Int. Ed. 2015, 54, 15565–15569; Optische Kontrolle der Insulinsekretion mit einem Inkretinschalter, Broichhagen, J.#; Podewin, T.#; Meyer-Berg, H.; von Ohlen, Y.; Johnston, N. R.; Jones, B. J.; Bloom, S. R.; Rutter, G. A.; Hoffmann-Röder, A.; Hodson, D. J.; Trauner, D., Angew. Chem. 2015, 127, 15786–15790.
11. Orthogonal optical control of a G protein-coupled receptor with a SNAP-tethered photochromic ligand, Broichhagen, J.#; Damijonaitis, A.#; Levitz, J.#; Sokol, K. R.; Leippe, P.; Konrad, D.; Isacoff, E. Y.; Trauner, D., ACS Cent. Sci. 2015, 1, 383–393.
10. A roadmap to success in photopharmacology, Broichhagen, J.; Frank, J. A.; Trauner D., Acc. Chem. Res. 2015, 48, 1947–1960.
9. Azobenzene-based inhibitors of human carbonic anhydrase II, Runtsch, L. S.; Barber, D. M.; Mayer, P.; Groll, M.; Trauner, D.*; Broichhagen, J.*, Beilstein J. Org. Chem. 2015, 11, 1129–1135.
8. Crystal structure of (E)-1,2-bis(4-bromo-2,6-difluorophenyl)diazene, Broichhagen, J.; Woodmansee, D. H.; Trauner, D.; Mayer, P., Acta Cryst. 2015, E71, o459–o460.
7. A red-shifted photochromic sulfonylurea for the remote control of pancreatic beta cell function, Broichhagen, J.; Frank, J. A.; Johnston, N. R.; Mitchell, R. K.; Smid, K.; Marchetti, P.; Bugliani, M.; Rutter, G. A.; Trauner, D.; Hodson, D. J., Chem. Commun. 2015, 51, 6018–6021.
6. AzoCholine enables optical control of alpha 7 nicotinic acetylcholine receptors in neural networks,Damijonaitis, A.; Broichhagen, J.; Hüll, K.; Nagpal, J.; Laprell, L.; Woodmansee, D. H.; Urushima, T.; Schönberger, M.; Rafiq, A.; Sumser, M. P.; Kummer, W.; Gottschalk, A.; Trauner, D., ACS Chem. Neurosci. 2015, 5, 701–707.
5. The in vivo chemistry of photochromic tethered ligands, Broichhagen, J.; Trauner, D., Curr. Opin. Chem. Biol. 2014, 21, 121–127.
4. Optical control of insulin release using a photoswitchable sulfonylurea, Broichhagen, J.; Schönberger, M.; Cork, S. C.; Frank, J. A.; Marchetti, P.; Bugliani, A. M.; Shapiro, A. M. J.; Trapp, S.; Rutter, G. A.; Hodson, D. J.; Trauner, D., Nat. Commun. 2014, 5, 5116.
3. Optical Control of Acetylcholinesterase with a Tacrine Switch, Broichhagen, J.; Jurastow, I.; Iwan, K.; Kummer, W.; Trauner, D., Angew. Chem. Int. Ed. 2014, 53, 7657-7660; Optische Kontrolle der Acetylcholinesterase mit einem schaltbaren Tacrin, Broichhagen, J.; Jurastow, I.; Iwan, K.; Kummer, W.; Trauner, D., Angew. Chem. 2014, 126, 7788–7792.
2. Bilirubin in a New Light, Broichhagen, J.; Trauner, D., Angew. Chem. Int. Ed. 2013, 52, 13868-13870; Bilirubin in einem neuen Licht, Broichhagen, J.; Trauner, D., Angew. Chem. 2013, 125, 14112–14114.
1. Strain-Promoted Alkyne Azide Cycloaddition for the Functionalization of Poly(amide)-Based Dendrons and Dendrimers, Ornelas, C.; Broichhagen, J.; Weck, M., J. Am. Chem. Soc. 2010, 132, 3923–3931.
# these authors contributed equally
* corresponding author
RESOURCES
EXTERNAL COLLABORATIONS
COURSES
tba.
MATERIAL
Do you want to try some material for your research. We believe in sharing compounds. Just get in contact!
CONTACT
Leibniz-Forschungsinstitut für
Molekulare Pharmakologie (FMP)
Robert-Roessle-Str. 10
13125 Berlin
Germany
+49–(0)30–94793–171
Describe your image